Codon Optimization to Enhance Expression Yields Insights into Chloroplast Translation
نویسندگان
چکیده
منابع مشابه
A role for initiation codon context in chloroplast translation.
To study the role of initiation codon context in chloroplast protein synthesis, we mutated the three nucleotides immediately upstream of the initiation codon (the -1 triplet) of two chloroplast genes in the alga Chlamydomonas reinhardtii. In prokaryotes, the -1 triplet has been proposed to base pair with either the 530 loop of 16S rRNA or the extended anticodon of fMet-tRNA. We found that in vi...
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The endosymbiosis of a free-living cyanobacterium into an ancestral eukaryote led to the evolution of the chloroplast (plastid) more than one billion years ago. Given their independent origins, plastid proliferation is restricted to the binary fission of pre-existing plastids within a cell. In the last 25 years, the structure of the supramolecular machinery regulating plastid division has been ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2016
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.16.00981